Prepacked HiTrap Capto HIC columns are also available individually. Capto HIC resins reduce process cycle times and increase throughput and productivity. These HIC resins are designed with a rigid matrix allowing high capacity, narrow specification range, high flow rate, and low backpressure. Capto HIC resins:Ĭapto HIC resins meet the modern demands of large-scale biopharmaceutical manufacturing and protein purification. HiTrap column format is designed for use with a syringe, peristaltic pump, or chromatography system such as an ÄKTA system. The Capto HIC selection kit, conveniently offered in ready-to-use HiTrap columns, enables you to test five different Capto HIC resins in order to find the most suitable resin and conditions for your purification process. Experimental parameters including type of salt, salt concentration, and pH may also be varied to produce the best purification conditions. For a given biomolecule, the strength of the hydrophobic interaction for HIC depends on the characteristic of both the biomolecule and the HIC resin. Resin selection and screening is an important part of HIC to determine optimal conditions for your target protein sample. The kit includes five 1 mL HiTrap columns: Narrow specification range ensures robust performance in sensitive processesĮxcellent chemical stability permits use of all standard cleaning-in-place (CIP) solutions, including 1 M sodium hydroxide Highly rigid matrix supports high flow rates, long bed heights, and faster throughput to promote scale-up, increase productivity, and improve process economy Prepacked, ready-to-use 1 mL columns offer ease and convenienceĬapto resins enable high-throughput chromatography for intermediate and polishing steps in downstream purification processes Resin variety allows for screening of selectivity, binding, and elution conditions: Capto Phenyl (high sub), Capto Phenyl ImpRes, Capto Butyl, Capto Butyl ImpRes, and Capto Octyl The HiTrap Capto HIC selection kit is useful in process development or research applications. The Capto hydrophobic interaction chromatography (HIC) selection kit has a set of five 1 mL ready-to-use HiTrap columns for resin screening and small-scale protein purification.
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